Structure and expression of [beta]-glucosidases and their binding proteins in Brassica napus L.

  • 2.54 MB
  • English
Uppsala Genetic Center, Dept. of Cell Research, Swedish University of Agricultural Sciences , Uppsala
Plant isozymes., Myrosinase., Glucosidases., Glucosinolates., Brassica -- Molecular gene
Statementby Anders Falk.
The Physical Object
Pagination1 v. (various pagings) :
ID Numbers
Open LibraryOL15398424M
ISBN 109157649006

Functional and Structural Diversity of Acyl-coA Binding Proteins in Oil Crops. (Brassica napus), sunflower structure, their binding affinity, their subcellular localization.

Introduction. Oilseed rape (Brassica napus L.) is one of the most important oil crops worldwide and provides high‐quality edible vegetable oil for human edibility and processing quality of rapeseed oil are mainly determined by the fatty acid composition of the seeds, particularly the proportions of the three major unsaturated fatty acids: oleic acid (C), linoleic acid Cited by: 2.

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Dynamics of protein expression during pollen germination in Canola (Brassica napus) Article in Planta (4) August with 30 Reads How we measure 'reads'. Diversities in structure and function of ACBP were discussed in this review.

ACBP are important proteins that could transport newly synthesized fatty acid, activated into -coA, from plastid to endoplasmic reticulum, where oil in the form of triacylglycerol occurs. ACBP were detected in various animal and plants species, which indicated their importance in biological by: 3.

Metabolic analysis in Brassica napus hypocotyl explants overexpressing AtRAP leads to wide metabolic changes. Interestingly, some of the metabolites that were consistently increased, such as γ-amino butyric acid and L-proline, have been brought into Author: Sutton Mooney, Raed Al-Saharin, Christina M.

Choi, Kyle Tucker, Chase Beathard, Hanjo A. Hellmann.

Description Structure and expression of [beta]-glucosidases and their binding proteins in Brassica napus L. FB2

The two major storage proteins identified in Brassica napus (canola) were isolated and studied for their molecular composition, structural characteristics and the responses of structural features to the changes in pH and temperature. Cruciferin, a complex of six monomers, has a predominantly β-sheet-containing secondary structure.

This protein showed low pH unstable tertiary structure, and Cited by: Genome-wide association study of heat stress-tolerance traits in spring-type Brassica napus L. under controlled conditions L. Taconnat, I. SmallGenome-wide analysis of Arabidopsis pentatricopeptide repeat proteins reveals their essential role in organelle biogenesis.

Plant Cell, 16 Cited by: 8. Abiotic stress greatly inhibits crop growth and reduces yields. However, little is known about the transcriptomic changes that occur in the industrial oilseed crop, rapeseed (Brassica napus), in response to abiotic stress. In this study, we examined the physiological and transcriptional responses of rapeseed to drought (simulated by treatment with 15% (w/v) polyethylene glycol (PEG) ) and Cited by: Structure and expression of [beta]-glucosidases and their binding proteins in Brassica napus L.

book. Rapeseed (Brassica napus L.) is an important oil crop in the world. In order to fulfill the requirement of mechanized harvesting and raised production, it is highly desirable to gain a better understanding of the regulatory networks controlling the main agronomic traits of this crop.

In this study, we obtained a natural mutant of rapeseed with more main stems named as dt (duo tou, meaning more Cited by: 1. Introduction. Canola (Brassica napus L.) is the member of the Brassicaceae family (syn. Cruciferae) and is the most commonly grown Brassica species in the –, the worldwide production of rapeseed oil was million metric tons (USDA Economics, ).Originally developed in Canada through breeding programs in the early 's (Przybylski et al.,Canola Council Cited by:   Abstract.

Low-molecular mass (10 kD) cytosolic acyl-coenzyme A-binding protein (ACBP) has a substantial influence over fatty acid composition in oilseeds, possibly via an effect on the partitioning of acyl groups between elongation and desaturation usly, we demonstrated that the expression of a Brassica napus ACBP (BnACBP) complementary DNA in the developing Cited by: Oilseed rape (Brassica napus L.) is an allotetraploid species with 2n = 38 chromosomes and two genomes (AA derived from B.

rapa and CC from B. oleracea).It is the most important source of edible vegetable oil and protein-rich meal in China. Rapeseed oil is similar to other vegetable : Min Yao.

Analysis of Self-Compatible Mutant BrassicaStrains. Two self-compatible (SC) mutant Brassica strains that exhibit defects in the structure or expression of SLG were used in this study: (a) Brassica campestris (syn.

rapa) strain homozygous for scf1, a recessive mutation at a trans-acting locus unlinked to the S locus that leads to a dramatic reduction in the levels of SLG transcripts and Cited by:   Rapeseed (Brassica napus L.) and soybean (Glycine max L.) seeds are rich in both protein and oil, which are major sources of biofuels and nutrition.

Although the difference in seed oil content between soybean (~ 20%) and rapeseed (~ 40%) exists, little is known about its underlying molecular mechanism. An integrated omics analysis was performed in soybean, rapeseed, Cited by: 1.

Brassica napus seed is a lipid storage organ containing approximately 40% oil, while its leaves contain many kinds of lipids for many biological roles, but the overall amounts are less than in seeds.

Thus, lipid biosynthesis in the developing seeds and the leaves is strictly regulated which results the final difference of lipids. However, there are few reports about the molecular mechanism Cited by: Rapeseed (Brassica napus) is an important oil seed crop, providing more than 13% of the world’s supply of edible oils.

An in-depth knowledge of the gene network involved in biosynthesis and accumulation of seed oil is critical for the improvement of B. napus. Using available genomic and transcriptomic resources, we identified 1, acyl-lipid metabolism (ALM) genes that are distributed over Cited by: In Brassica napus, oleosins are expressed at high levels in the seed during the latter stages of embryo development.

The cis-acting regulatory properties of an bp promoter fragment of a B. napus oleosin gene were examined by analysis of beta-glucuronidase (GUS) expression in transgenic tobacco plants containing an oleosin promoter-GUS transcriptional by: Tang XC, He YQ, Wang Y, Sun MX () The role of arabinogalactan proteins binding to Yariv reagents in the initiation, cell developmental fate, and maintenance of microspore embryogenesis in Brassica napus L.

Topas. J Exp Bot – PubMed Google ScholarCited by: 2. Identification and comparative expression analysis of odorant binding protein genes in the tobacco cutworm Spodoptera litura Identification and expression of odorant-binding proteins of the malaria-carrying mosquitoes Anopheles gambiae and Anopheles arabiensis.

Cloning and functional characterization of a Brassica napus transporter that Cited by:   The Cdc20 and Cdh1/Fzr proteins are the substrate-specific activators of the anaphase-promoting complex (APC).

In Medicago truncatula, the MtCcs52A and MtCcs52B proteins represent two subgroups of the Cdh1-type activators, which display differences in their cell cycle regulation, structure, and function. The ccs52A transcripts are present in all phases of the cell by:   Oilseed rape (Brassica napus L.), which has yellow flowers, is both an important oil crop and a traditional tourism resource in China, whereas the Orychophragmus violaceus, which has purple flowers, likely possesses a candidate gene or genes to alter the flower colour of oilseed rape.A previously established B.

napus line has a particular pair of O. violaceus chromosomes (M4) and exhibits Cited by: 4. AGL15 (for AGAMOUS-Like 15) is a member of the MADS domain family of DNA binding transcriptional regulators that accumulates to its highest amounts during embryo development. To better understand how AGL15 functions, a chromatin immunoprecipitation approach was used to identify directly regulated genes.

One DNA fragment that coprecipitated with AGL15 corresponded to a portion of the Cited by:   Seed storage protein gene promoters contain conserved motifs. Seed storage protein gene promoter sequences (the bp upstream region of the transcriptional start) from Brassicaceae (15 promoters), Fabaceae (17 promoters) and Poaceae (22 promoters) were retrieved from public sequence databases.

Discriminative seeding DNA motif discovery [] was performed separately in each of the Cited by: In Brassica napus, yellow petals had a much higher content of carotenoids than white petals present in a small number of lines, with violaxanthin identified as the major carotenoid compound in yellow petals of rapeseed lines.; Using positional cloning we identified a carotenoid cleavage dioxygenase 4 gene, 4, responsible for the formation of flower colour, with preferential expression Cited by: Identification and expression profiling of odorant binding proteins and chemosensory proteins between two wingless morphs and a winged morph of the cotton Cited by:   Gene synteny analysis revealed higher gene copy number in B.

napus. In total, genes related to FA and TAG biosyntheses were identified in the genome of B. napus, they were homologous toand genes from A. thaliana, B. rapa and B. oleracea, B. napus, A n and C n genomes contained and genes, respectively (Table 1; Additional file 1: Table S1).Cited by: 6.

Plants require sunlight, water, CO2, and essential nutrients to drive photosynthesis and fulfill their life cycle. The photosynthetic apparatus resides in chloroplasts and fundamentally relies on transition metals as catalysts and cofactors.

Accordingly, chloroplasts are particularly rich in iron (Fe), manganese (Mn), and copper (Cu). Owing to their redox properties, those metals need to be. MicroRNAs (miRNAs) are noncoding RNAs that play an important role in the regulation of the genetic expression in animals and plants by targeting mRNAs for cleavage or translational repression.

Several miRNAs regulate the plant development, the metabolism, and the responses to biotic and abiotic stresses.

Characterization of an miRNA has helped to show its role in fine tuning the mechanisms of Author: Vargas-Hernández Marcela, Vázquez-Marrufo Gerardo, Aguilar-Ruiz Carlos Agustín, González-Márquez Mar. Towards map-based cloning: fine mapping of a recessive genic male-sterile gene (BnMs2) in Brassica napus L.

and syntenic region identification based on the Arabidopsis thaliana genome sequences. Source: THEORETICAL AND APPLIED GENETICS: Year. Seeds of B. napus and R.

Details Structure and expression of [beta]-glucosidases and their binding proteins in Brassica napus L. FB2

communis were collected from greenhouse‐grown plants (15 h photoperiod; 22 and 28°C, respectively). Brassica napus flowers were tagged on the first day after flowering (DAF) and collected at four developmental stages, 12–20, 21–25, 26–30, and 31–35 by:.

Brassica napus L. is an important oilseed and fodder crop with significant heterosis for seed yield and other agronomic traits, but very little is known about the molecular basis of heterosis. As an initial step towards understanding the molecular events associated with this phenomenon, a molecular functional map of rapeseed was constructed using differentially expressed genes in hybrid.Enter search terms.

Keep search filters New search. Advanced search.Higher expression of genes encoding microtubule-associated protein and as well as tubulin subunits γ-1, β-1, β-3 and microtubule motor activity proteins belonging to kinesin family was also observed during PEG soaking and in dry osmoprimed Brassica napus by: